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We would like to thank Prof. First, it can screen nearly every restriction site at the whole genome level whereas other RAD-Seq methods can only get a subset of total sites due to the multiple time size selection for efficient PCR amplification and sequencing. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. The male-specific tags were then mapped to a female bighead carp genome Shunping He, unpublished data , and those tags failing to align to the female reference genome were considered as putative male-specific tags. However, unlike the mammals or birds with significant difference in sex chromosomes, large differentiated genomic regions are uncommon in fish. The 16 silver carp samples used to verify the markers were sampled from Shishou Fish Farm Shishou, China , and they were caught from the Yangtze River when they were young and then reared in the farm until sexually mature. This strategy of SCAR-based PCR detection in this study is the first protocol successfully amplifying the Y-specific fragment in bighead carp and silver carp. The PCR results from different sex with these primers further identified that this region is male-specific region in the bighead carp genome Fig.

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